Blue®Plate

Cell Wash for Flow Cytometry

Blue®Plate enables fully automated, high-throughput cell washes for flow cytometry with minimal cell loss or damage and highly effective background removal

Low-g, gentle centrifugation deposits cells into Cell®Safe reservoirs at the bottom of Blue®Plate wells where they are safely retained while the Blue®Washer centri- fugally evacuates all of the liquid above the reservoirs. The Blue®Washer dispenser efficiently re-suspends cells for subsequent washes.

Available for 96w plates, the method is applicable to all plate and tube formats.

Fast

<4MIN PER WASH CYCLE 
AUTOMATION FRIENDLY

150 sec: Spin (low-g) cells into Cell®Safe reservoirs
30 sec: Remove all medium above Cell®Safe reservoirs
15 sec: Resuspend cells
Can be fully automated
One Blue®Washer instrument to wash both suspension and adherent cells

GENTLE

MINIMAL CELL LOSS, 
DAMAGE OR ACTIVATION

Gently spinning (<30g)
cells into Cell®Safe reservoirs ensures cells do not get harmed or activated and do not clump
>90% cell retention per wash step

EFFECTIVE

EFFECTIVE BACKGROUND REMOVAL GENERATES HIGH QUALITY, REPRODUCIBLE FLOW DATA

40x dilution per wash step due to 5ul residual in Cell®Safe reservoirs vs 200ul wash buffer capacity in well above
High speed re-suspension of cells – not pellets - efficiently removes non-specific binding
100% reproducible workflow - low %cv
Fully automated – same results across operators and sites
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Blue®Plate

FOR FLOW CYTOMETRY AUTOMATED WORKFLOW

1

Spin cells into
 Cell®Safe

High-Throughput Mode: <30 sec
High-Retention Mode: 3 min

Load Blue®Plate into conventional centrifuge or the Blue®Washer with Blue®Plate inserted upside down 

Low-g, gentle centrifugation deposits cells into Cell®Safe reservoirs at the bottom of  Blue®Plate (10-30g, 2-4min)

2

remove all medium above Cell®Safe

30 sec

Cell®Safe reservoirs at the bottom of Blue®Plate wells safely retain cells while the Blue®Washer centrifugally evacuates all of the liquid above the reservoirs

3

re-suspend cells 
out of Cell®Safe

15 sec

The Blue®Washer dispenser quickly re-suspends cells for subsequent washes,
efficiently mixing them with wash buffer

Specifications

Name
BluePlate 96Well
Product Number
BP96C
Material
Polypropylene
Working Volume
200 μl
Volume Cell®Safe
5 μl

Blue®Plate

Blue®Plate enables fully automated, high-throughput cell washes for flow cytometry
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Set of 2 carriers with 52mm cut-out compatible with HRB grippers
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Cleaning solution for the daily cleaning procedure of rotor and rotor chamber
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Blue® Intense
Cleaning solution to avoid corrosion and extend the lifetime of rotor and rotor chamber
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Test your customized Blue®Washer configuration

Each assay is different! Let us know about your specific workflows & individual targets to get a customized Blue®Washer configuration for your assay campaigns.
GET FREE DEMO
2D Cellular Assays
3D Cellular Assays
Suspension Cells
Cell Seeding
Magnetic Bead Assays*
Genomics - NGS Assays*
Dispensing
Plate Coating
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