Flow Cytometry

Blue®Plate enables fully automated, high-throughput cell washes for flow cytometry with minimal cell loss or damage and highly effective background removal. Low-g, gentle centrifugation deposits cells into Cell®Safe reservoirs at the bottom of Blue®Plate wells where they are safely retained while the Blue®Washer centrifugally evacuates all of the liquid above the reservoirs. The Blue®Washer dispenser efficiently re-suspends cells for subsequent washes.

Blue®Plate

FOR FLOW CYTOMETRY AUTOMATED WORKFLOW

1

Spin cells into
 Cell®Safe

High-Throughput Mode: <30 sec
High-Retention Mode: 3 min

Load Blue®Plate into conventional centrifuge or the Blue®Washer with Blue®Plate inserted upside down 

Low-g, gentle centrifugation deposits cells into Cell®Safe reservoirs at the bottom of  Blue®Plate (10-30g, 2-4min)

2

remove all medium above Cell®Safe

30 sec

Cell®Safe reservoirs at the bottom of Blue®Plate wells safely retain cells while the Blue®Washer centrifugally evacuates all of the liquid above the reservoirs

3

re-suspend cells 
out of Cell®Safe

15 sec

The Blue®Washer dispenser quickly re-suspends cells for subsequent washes,
efficiently mixing them with wash buffer
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Application Notes

19. August 2024

HLA microarray production using Blue®Washer

Blue®Washer Optimizes HLA Microarray Production with High Spot Integrity

The Authors evaluated the Blue®Washer for the production of HLA microarrays, demonstrating its compatibility by "maintaining spot integrity and sensitivity at low (35-50g) g-forces" during washes. Blue®Washer accelerates and simplifies the manufacturing process, making it suitable for "high-throughput production while preserving the quality" of the microarrays.

Download Paper

6. September 2021

Plate Coating Protocol

Knight al al. (2018) desribe  a coating protocol for 1536 MTPs using BlueWasher and achieve ready to go plates for cellular assays. Using a dynamic mass distribution (DMR) assay they could show an improvement of z' - value of about 1.0 due to removal of resdual fibronectin with the BlueWasher.

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Test your customized Blue®Washer configuration

Each assay is different! Let us know about your specific workflows & individual targets to get a customized Blue®Washer configuration for your assay campaigns.
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2D Cellular Assays
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Suspension Cells
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Genomics - NGS Assays*
Dispensing
Plate Coating
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